Abstract
The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays.
Highlights
IntroductionThe funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
We show that the cytoplasmic chemoreceptor of the Frz chemosensory system, FrzCD, does not bind the cytoplasmic membrane like most Methyl-accepting Chemotaxis Proteins (MCP) but bind the bacterial nucleoid directly, forming distributed protein clusters containing the FrzE kinase
This work shows the importance of the nucleoid in the organization and functioning of cytoplasmic signaling systems in bacteria
Summary
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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