Abstract

The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays.

Highlights

  • IntroductionThe funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

  • We show that the cytoplasmic chemoreceptor of the Frz chemosensory system, FrzCD, does not bind the cytoplasmic membrane like most Methyl-accepting Chemotaxis Proteins (MCP) but bind the bacterial nucleoid directly, forming distributed protein clusters containing the FrzE kinase

  • This work shows the importance of the nucleoid in the organization and functioning of cytoplasmic signaling systems in bacteria

Read more

Summary

Introduction

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Methods
Results
Discussion
Conclusion
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.