The nuclear matrix is involved in herpes simplex virogenesis

Abstract

Nuclear matrices have been purified from BHK cells infected with herpes simplex virus type 1 (HSV-1) and compared with nuclear matrices from uninfected BHK cells. Both showed typical structure with residual nucleolus, peripheral lamina with pore complexes, and a nonchromatin fibrillar network. Numerous viral capsids were seen stuck to that framework in matrices from infected cells. SDS-PAGE and fluorography analysis showed that polypeptides normally found in the noninfected BHK nuclear matrix were still present in the infected matrices. Nine additional virus-induced polypeptides were detected 15 hr after infection by labeling with [ 35S]methionine. This suggests that the nuclear matrix is involved in the formation of the capsid or in the encapsidation process. Twelve DNA-binding proteins were also detected in infected matrices.