The nuclear immune receptor RPS4 is required for RRS1SLH1-dependent constitutive defense activation in Arabidopsis thaliana.

Kee Hoon Sohn,Toby E Newman,Cécile Segonzac,Simon J Williams,Bostjan Kobe,Jonathan D G Jones,Kyung Hee Paek,Ghanasyam Rallapalli,Joo Yong Woo,Panagiotis F Sarris,Jeffery L Dangl

doi:10.1371/journal.pgen.1004655

Abstract

Plant nucleotide-binding leucine-rich repeat (NB-LRR) disease resistance (R) proteins recognize specific “avirulent” pathogen effectors and activate immune responses. NB-LRR proteins structurally and functionally resemble mammalian Nod-like receptors (NLRs). How NB-LRR and NLR proteins activate defense is poorly understood. The divergently transcribed Arabidopsis R genes, RPS4 (resistance to Pseudomonas syringae 4) and RRS1 (resistance to Ralstonia solanacearum 1), function together to confer recognition of Pseudomonas AvrRps4 and Ralstonia PopP2. RRS1 is the only known recessive NB-LRR R gene and encodes a WRKY DNA binding domain, prompting suggestions that it acts downstream of RPS4 for transcriptional activation of defense genes. We define here the early RRS1-dependent transcriptional changes upon delivery of PopP2 via Pseudomonas type III secretion. The Arabidopsis slh1 (sensitive to low humidity 1) mutant encodes an RRS1 allele (RRS1SLH1) with a single amino acid (leucine) insertion in the WRKY DNA-binding domain. Its poor growth due to constitutive defense activation is rescued at higher temperature. Transcription profiling data indicate that RRS1SLH1-mediated defense activation overlaps substantially with AvrRps4- and PopP2-regulated responses. To better understand the genetic basis of RPS4/RRS1-dependent immunity, we performed a genetic screen to identify suppressor of slh1 immunity (sushi) mutants. We show that many sushi mutants carry mutations in RPS4, suggesting that RPS4 acts downstream or in a complex with RRS1. Interestingly, several mutations were identified in a domain C-terminal to the RPS4 LRR domain. Using an Agrobacterium-mediated transient assay system, we demonstrate that the P-loop motif of RPS4 but not of RRS1SLH1 is required for RRS1SLH1 function. We also recapitulate the dominant suppression of RRS1SLH1 defense activation by wild type RRS1 and show this suppression requires an intact RRS1 P-loop. These analyses of RRS1SLH1 shed new light on mechanisms by which NB-LRR protein pairs activate defense signaling, or are held inactive in the absence of a pathogen effector.

Highlights

Plant innate immunity relies on two layers of pathogen detection
We show an auto-active allele of RRS1, RRS1SLH1, triggers transcriptional reprogramming of defense genes that are reprogrammed by AvrRps4 or PopP2. Pto DC3000 (PopP2) in an RPS4/RRS1-dependent manner
We found that most (,83%) of the PopP2/RRS1 effector-triggered immunity (ETI) genes were differentially expressed in slh1 auto-immune and temperature shift responses, while up to 54% of ETI genes were differentially expressed in the auto-immune response but not by temperature shift (Figure 4, black box)

Summary

Introduction

Plant innate immunity relies on two layers of pathogen detection. Cell surface-localized pattern recognition receptors detect pathogen-associated molecular patterns (PAMPs) of invading microorganisms and activate PAMP-triggered immunity (PTI) [1]. Effectors can be detected directly or indirectly by plant disease resistance (R) proteins, which activate effector-triggered immunity (ETI) generally together with a hypersensitive response (HR) of the infected tissue [2]. Some NB-LRR proteins carry an additional carboxyl-terminal extension, the function of which is unknown [3]. NB-LRR protein function generally requires an intact P-loop motif (GxxxxGKT/S) in the NB domain, presumably for ATP binding and energy-dependent conformational changes [3,4]. Plant NB-LRR proteins and mammalian Nod-like receptors (NLRs) exhibit both structural and functional similarities [5]

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