The N-terminal polypeptide derived from vMIP-II exerts its anti-tumor activity in human breast cancer by regulating lncRNA SPRY4-IT1.

Haihua Wu,Sulian Chen,Haifeng Wang,Tiantian Chen,Yueyue Wang,Qingling Yang,Changjie Chen,Yu Li,Lingyu Zhang,Wenrui Wang

doi:10.1042/bsr20180411

Abstract

Accumulating evidence demonstrates that long non-coding RNA (lncRNA) sprouty4-intron transcript 1 (lncRNA SPRY4-IT1) plays a vital role in the development of breast cancer. However, the underlying mechanism has not been eventually illuminated. We aimed to explore the biological activity of lncRNA SPRY4-IT1 in breast cancer cells and whether N-terminal polypeptide derived from viral macrophage inflammatory protein II (NT21MP) could exert its anti-tumor effect by regulating lncRNA SPRY4-IT1 and its target gene SKA2. Real-time RT-PCR, Western blotting, wound healing, and invasion assays were used to achieve this goal. We found that lncRNA SPRY4-IT1 was highly expressed in breast cancer cells. Moreover, NT21MP markedly inhibited biological effects of breast cancer cells by regulating lncRNA SPRY4-IT1, which was partially achieved through SKA2. Our findings suggested that lncRNA SPRY4-IT1 could serve as a novel biomarker by NT21MP for breast cancer.

Highlights

Breast cancer is one of the most common malignant tumors around the world, which is a serious threat to women’s physical and mental health [1,2]
In the pcDNA-CXC chemokine receptor 4 (CXCR4) group, the expression of SPRY4-IT1 was not statistically significant compared with the control group due to low expression of stromal cell-derived factor 1-alpha (SDF-1α)
These results showed that the expression of SPRY4-IT1 was related to SDF-1α/CXCR4 axis

Summary

Introduction

Breast cancer is one of the most common malignant tumors around the world, which is a serious threat to women’s physical and mental health [1,2]. In the past few years, treatments of breast cancer have made great progress, the features of cell proliferation, invasiveness, and metastasis [5,6] are still the main reason leading to higher mortality amongst breast cancer patients, which seriously affects the patients’ prognosis. These mechanisms are quite complex and involve many biological processes. Long non-coding RNA (lncRNA) is a class of functional RNA molecules with a length of more than 200 nts, which do not have the ability to encode proteins It plays an important role in the regulation of epigenetic, transcription, and post-transcriptional levels [7]. The underlying mechanism of lncRNA SPRY4-IT1 in breast cancer remains unclear

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Results

Conclusion