The NQO1 allelic frequency in hindu population of central India varies from that of other Asian populations

Abstract

CONTEXT:The enzymes encoded by the polymorphic genes NAD (P) H: quinone oxidoreductase 1 (NQO1) play an important role in the activation and inactivation of xenobiotics. This enzyme has been associated with xenobiotic related diseases, such as cancer, therapeutic failure and abnormal effects of drugs.AIM:The aim of the present study was to determine the allelic and genotypic frequencies of NQO Hinf I polymorphisms in a Hindu population of Central India.SETTINGS AND DESIGN:Polymorphisms of NQO1 were determined in 311 unrelated Hindu individuals.MATERIALS AND METHODS:Polymerase chain reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) analysis in peripheral blood DNA for NQO1 Hinf I polymorphism was used in 311 unrelated Hindu individuals.STATISTICAL ANALYSIS:Allele frequencies were calculated by direct counting. Hardy Weinberg Equilibrium was evaluated using a Chi-square goodness of fit test.RESULTS:The observed allelic frequency was 81% for C (wild) and 19% for T (mutant) in the total sample.CONCLUSIONS:The allelic frequency of “C” was higher than in other Asians (57%), but similar to Caucasians (81%). The genotype distributions for Hinf I polymorphisms were in Hardy-Weinberg equilibrium.