The novel few layered MXene/MoS2/PdPtNPs nanomaterials combined with hybrid chain reaction for electrochemical detection of mucin 1

Abstract

This work has developed a novel and highly sensitive electrochemical aptasensor for the detection of mucin 1 (MUC1) based on the MXene/MoS2/PdPtNPs nanomaterials and hybridization chain reaction (HCR). MXene/MoS2/PdPtNPs nanomaterials were first prepared via a simple hydrothermal and external reduction method, which was used as the supporting substrate of the biosensor. MXene synergized with MoS2-PdPtNPs could prevent the few layered MXene from curling effectively. Besides, the proposed sensing platform could simultaneously increase the active site and enhance the electrochemical performance. In the presence of MUC1, dsDNA structural switching was induced by MUC1 binding to aptamer specifically, and most aptamers were pulled off from the electrode surface, which thus resulted in the assembly of initiator S, harpin probe 1 (H1) and harpin probe 2 (H2) on electrode surface to trigger the HCR. Since the peroxidase-like mimic manganese (III) meso-tetrakis (4-Nmethylpyridyl)-porphyrin (MnTMPyP) interacts with DNA nanoladders via groove binding, the combined PdPtNPs could result in a great current response to the hydrogen peroxide hydroquinone system. For MUC1 detection, the proposed biosensor has showed a wide linearity range of 1 pg mL−1–10 ng mL−1 and a low detection limit of 0.36 pg mL−1 (S/N = 3), which was hopeful to be applied in biotechnology and clinical diagnosis.