The novel candidate gene Grb10 regulates the growth performance of Guizhou white goats

Abstract

Growth factor receptor binding protein 10 (Grb10) regulates muscle growth but its function in goats is not well studied. This study examined the relationship between Grb10 gene polymorphism and growth traits in Guizhou white goats. Further, the effect of promoter polymorphism on Grb10 transcription was analyzed via a dual-luciferase assay. Quantitative reverse transcription-polymerase chain reaction analysis showed that the expression level of the Grb10 gene was the highest in the longissimus dorsi muscle (P < 0.01) and the lowest in the spleen. Moreover, sequencing results showed that the Grb10 gene had five single nucleotide polymorphisms. The association analysis showed that g.-246 C > T, g.72323T > C, and g.178634A > G loci were significantly associated with growth traits (P < 0.05). Linkage disequilibrium analysis of each locus showed that g.72323T > C and g.72814T > A loci had the strongest linkage (r2 = 0.482, D' = 0.73). Furthermore, according to an analysis conducted using TFDB4 software, it was predicted that a mutation in the promoter region of the g.-246 C > T locus resulted in the absence of four transcriptional regulatory factors. The results of dual-luciferase reporter assay further demonstrated that mutation of the g.-246 C > T locus reduced the transcriptional activity of the promoter region of the Grb10 gene. These findings suggest that Grb10 gene polymorphism could affect growth traits in goats.