The NLRP3 Inflammasome and Diabetic Cardiomyopathy

Abstract

Chronic hyperglycemia and dyslipidemia are primarily linked to various complications of diabetes, especially of type 2. Diabetes mellitus, both type 1 and 2, accompanies cellular oxidative/nitrosative stress and insulin resistance/deficiency leading to generation of a chronic low-grade inflammatory environment within tissues. Recent studies have focused on the role of a protein platform called the NLRP3 inflammasome ((nucleotide-binding domain, leucine-rich repeats containing family, pyrin domain-containing-3 (NLRP3), its adaptor apoptotic speck protein containing a caspase recruitment domain (ASC) and pro-caspase-1)), which activates caspase-1 and interleukin-1beta (IL-1β) [1–3]. Among the various inflammasomes, NLRP3 is widely activated in sterile inflammation (inflammation not involving infection) and is the most studied inflammasome. IL-1β is a highly inflammatory cytokine; therefore, its activity is regulated at multiple steps, including priming (expression), processing (activation) and exocytosis (release) [4]. Subsequently, pro-inflammatory IL-1β acts on its surface receptor, IL-1R, which may activate its own expression and other inflammatory cytokines generating reactive oxygen/nitrogen species (ROS/RNS) and cellular stress. IL-1β itself may propagate a feed forward loop maintaining chronic ROS/RNS stress, inflammation and premature cell death and disease progression of diabetes, including diabetic cardiomyopathy (DCM) (Fig. 1). As mentioned above, the NLRP3 inflammasome is considered as a sensor of cellular stress induced by various extrinsic (bacteria, virus, toxins, UV, and others) and intrinsic (metabolic imbalance, mitochondrial and endoplasmic reticulum stresses, organelle damage and oxidative stress) agents. Therefore, understanding the molecular mechanism(s) of the NLRP3 inflammasome assembly and activation is critical and currently constitutes an area of intense research in chronic metabolic diseases such as diabetes and obesity. In this issue of Cardiovascular Drugs and Therapy, an article by Beibei et al. provides the first report that the NLRP3 inflammasome components are activated in a model of type 2 diabetes in rats and that NLRP3 knock down by microRNA in vivo ameliorates cardiac remodeling and dysfunction [5]. In addition, rosuvastatin (RSV), a statin inhibiting the HMG-CoA enzyme, blocks excessive expression of the thioredoxin interacting protein (TXNIP), a protein that is considered to be involved in cellular oxidative stress and the NLRP3 inflammasome assembly [1–3], and also showed that RSV reduces the level of NLRP3, caspase-1, and IL-1β. RSV further suppresses activation of mitogen-activated protein kinases (MAPKs) such as ERK/p38 MAPK and JNK in this type 2 diabetes rat model. Nonetheless, a direct role of TXNIP in NLRP3 inflammasome assembly is yet to be fully understood [6] while TXNIP is known to cause pro-IL-1β and NLRP3 gene expression via nuclear factor NF-κB activation [7, 8]. Moreover, the long-term use of RSV in diabetics may have deleterious side effects. The question then remains which of these molecules and signaling pathways generating oxidative stress and the low-grade inflammation (such as TXNIP, NLRP3, caspase-1, MAPK/NF-κB, IL-1β itself or its receptor IL-1R) are the best targets to treat chronic metabolic diseases. Whether anti-oxidant and anti-inflammatory drugs should be introduced as adjuvant therapies in type 1 and 2 diabetes is still debated. Several trials are also being conducted using neutralizing antibodies targeting both IL-1β and its receptor IL-1R in various chronic metabolic and inflammatory diseases [9–11]. L. P. Singh (*) Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA e-mail: plsingh@med.wayne.edu