The next-generation sequencing reveals the complete mitochondrial genome of Alosa sapidissima (Perciformes: Clupeidae) with phylogenetic consideration

Jing Wang,Zeshu Yu,Xiang Wang,Shaosheng Yang,Dongguo Zhang,Yong Zhang

doi:10.1080/23802359.2017.1331322

Jing Wang, Zeshu Yu + Show 4 more

Open Access

https://doi.org/10.1080/23802359.2017.1331322

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Journal: Mitochondrial DNA Part B	Publication Date: Jan 1, 2017
Citations: 3	License type: open-access

Affiliation: Sun Yat-sen University

Abstract

The complete mitochondrial genome of the Alosa sapidissima is presented in this study. The mitochondrial genome is 16,741 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of Epinephelus awoara mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand in descending order is 28.79% of G, 28.39% of T, 24.94% of A, and 17.88% of C. With the exception of the NADH dehydrogenase subunit 6 (ND6) and 8 tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method showed that the A. sapidissima has the closer relationship to between the A. alosa and A. pseudoharengus in the phylogenetic relationship.

Highlights

The American shad, Alosa sapidissima, is a eurythermic migratory fish which can survive in fresh and brackish water
Alosa sapidissima is rich in protein and unsaturated fatty acids, contains calcium, iron, phosphorus, and magnesium; its docosahexenoic acid (DHA) content is much higher than the general freshwater fish
Most of the genes are encoded on the heavy strand, with only the NADH dehydrogenase subunit 6 (ND6) and eight transfer RNA genes (tRNA) genes [Gln, Ala, Asn, Cys, Try, Glu, Pro, Ser (TGA)] encoded on the light strand

Summary

Introduction

KEYWORDS Alosa sapidissima; mitochondrial genome; phylogenetic analysis The American shad, Alosa sapidissima, is a eurythermic migratory fish which can survive in fresh and brackish water. Alosa sapidissima is rich in protein and unsaturated fatty acids, contains calcium, iron, phosphorus, and magnesium; its docosahexenoic acid (DHA) content is much higher than the general freshwater fish. The next-generation sequencing (NGS) technologies, such as Illumine, allow considerable numbers of sequence data to be rapidly and efficiently characterized, which make it feasible for mitogenomes (Gilbert et al 2007).

Results

Conclusion