Abstract
The new method of restoration of injured articular cartilage using plasty of intraoperative bone-cartilage defects with combined allograft based on biologic porous carrier and cell culture from stroma of costal cartilage. The transplantation of allogenic culture of chondroblast cells on demineralized spongy substance «Lioplast» ensure еру the formation of hyaline cartilage tissue on the site of defect and amplified development of microcirculatory network in subchondral bone. The heterotopic principle obtaining cellular material allows to perform low-traumatic operations.
Highlights
The new method of restoration of injured articular cartilage using plasty of intraoperative bone-cartilage defects with combined allograft based on biologic porous carrier and cell culture from stroma of costal cartilage was developed
The transplantation of allogenic culture of chondroblast cells on demineralized spongy substance «Lioplast» ensure the formation of hyaline cartilage tissue on the site of defect and amplified development of microcirculatory network in subchondral bone
5. Êîñòíî-õðÿùåâîé äåôåêò áåç çàïîëíåíèÿ òðàíñïëàíòàòîì, 6 ìåñÿöåâ ýêñïåðèìåíòà: ñóñòàâíàÿ ïîâåðõíîñòü äåôîðìèðîâàíà, òóñêëàÿ, âèçóàëèçèðóþòñÿ óãëóáëåíèÿ â îáëàñòè ìûùåëêîâ áåäðåííîé êîñòè âèçÂóíàëóüòíðîèñíóåñâòûàâÿíâûëÿåëñèï.àÀéìêèïëíèàòóääààíäíâûèõæñåðíîèêéàõâ îãâðîïóéåïðâïèåêðîîâíâîàòíðâàñíëåûõàõòñåêëðîóàë÷ëåàüíÿíõíûûõíõåñóñîñóòòñëàòèâà÷àâàõàë.õàñâüîîïòûòòàíêîîéðåçÒóëàêüòèàìòûîáñðâàèçäîåìò,åëïüîñëòóâ÷îåâíàíëûè åî âáåýçêîñïïàåñðíèîìñåòíèòèå ýëôåíôèåÿêòäèåôâíåîêñòòîèâïñðóåñäòëàâîíæîåãíîíãîèãàîëìèåíòîîâäîàãâîîõñðñÿòàùíàî.â-
Summary
The new method of restoration of injured articular cartilage using plasty of intraoperative bone-cartilage defects with combined allograft based on biologic porous carrier and cell culture from stroma of costal cartilage was developed. ÕìÏîîñíääïðîãàåìëêîüñùíåíîüàéþëêîçîóâñáûòîìèââðàëà÷èåá2íöîèãîëèáíîðäàðèï÷ðåèñêìèàõëûêõîñîòáíîîð-îõòðàÿõùôåîâðûìõèðäîå-ôùãëååóêëáòêèàîíââîéîáá5åëäìàðñìåò.íèíâîíéóòêðîåñíòíèåãäîèèàìíåàòððóîæìíî2ã,î5 ììûì-, ýêñÏïåððåèäìâàåðíèòòàåëëüüííûîåóãð7óêïðïîûëè, îêñîóâù, íååñòââõëîÿäëÿèùâèçõÿâòèå ðåáåðíîãî õðÿùà äëÿ ïîëó÷åíèÿ àëëîãåííîãî êëåòî÷íîãî ìàòåðèàëà. Âûðàùèâàíèå ôèáðîáëàñòîïîäîáíûõ êëåòîê ïðîèçâîäèëè â êóëüòóðàëüíûõ ôëàêîíàõ «Ñorning» â òåðìîñòàòå «Sanio-Incubator» MIR ïðè òåìïåðàòóðå +37°Ñ Âûáîð íàìè äàííîãî àëëîãåííîãî êëåòî÷íîãî ìàòåðèàëà â ñîñòàâå êîìáèíèðîâàííîãî òðàíñïëàíòàòà áûë îáóñëîâëåí îñîáåííîñòÿìè õðÿùåâîé òêàíè, åå ñëàáîé àíòèãåííîñòüþ.
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