Abstract
In this study bacterial contamination of corn grains for ethanol fermentation was tested. Microbial pollution is a major problem for distilleries all over the world. The aim of this work was to verify whether chemical agents such as ammonia and propionic acid are efficient in reduction of undesirable microflora in grains. The number of total viable bacteria, lactic acid bacteria, acetic acid bacteria, anaerobic bacteria and the quantity of yeasts and moulds were signified by plate method. The ethanol yield was determined with the use of HP 5890 (Hewlett Packard, USA) gas chromatograph. It occurred that both agents effectively reduce bacterial contamination of corn grains, but fermentation process of propionic acid treated grains no longer proceeds. Thus, ammonia solution is as better disinfection agent for grains used as a raw material for bioethanol production. It may be also useful in disinfection other raw materials to new fermentation technologies which do not require former heating.
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