The neurons in mouse V1 show different degrees of spatial clustering

Abstract

Higher mammals’ primary visual cortex exhibits columnar organization, where neurons with similar response preferences are clustered. In contrast, rodents are presumed to lack this fine-scale organization; their neurons appear to be randomly arranged, described as a salt-and-pepper map. However, recent studies suggested a weak but significant spatial clustering of tuning in the salt-and-pepper map, similar to columnar organization. Thus, the salt-and-pepper map possesses the characteristics of both columnar organization and random arrangement. This raises the question about whether this mixed organization is attributed to different types of neurons. Here, we examined the tuning of primary visual cortical neurons in awake mice with a two-photon calcium imaging dataset, which were released by Allen Institute MindScope Program. First, we demonstrated that neurons with similar response preferences were clustered by showing that neighboring neurons tended to have similar orientation and temporal frequency preferences. Then, we compared the clustering of tuning between simple cells and complex cells and found the clustering of tuning among simple cells was significantly more prominent than that among complex cells. Furthermore, the simple/complex cell classification correlated with the stability of neuronal response. Neurons with stable responses were arranged independent of their tuning similarity, whereas unstable neurons were clustered according to their tuning similarity. These findings might represent a balance between efficiency and robustness: relatively independent tuning among stable neurons represents visual information efficiently, whereas unstable neurons with similar response preferences are clustered to obtain a robust representation with population codes.