Abstract
Cerebrospinal fluid-touching neurons (CSF-cNs) exist in the region surrounding the central canal of the spinal cord, which locate in the adult neurogenic niche. Previous research showed that CSF-cNs expressed the molecular markers of immature neural cells in vivo. Here, we explored the potential of CSF-cNs as neural stem cell in intro. We first found that PKD2L1+ CSF-cNs, isolating by FACS using the molecular marker PKD2L1 of CSF-cNs, expressed neural stem cells markers like Nestin, Sox2, and GFAP by immunofluorescence staining. PKD2L1+ CSF-cNs were able to form neurospheres and passaged in vitro. Immunofluorescence staining showed that the neurospheres forming by PKD2L1+ CSF-cNs also expressed neural stem cell markers Nestin, Sox2 and GFAP. The neurospheres expressed proliferation markers Ki67 and PCNA by immunofluorescence staining, indicating that the neurospheres forming by PKD2L1+ CSF-cNs were proliferative. The neurospheres, forming by CSF-cNs, had the ability of differentiation into neurons, astrocytes, and oligodendrocytes. Collectively, our data suggested that PKD2L1+ CSF-cNs have the properties of neural stem cells in vitro and may provide a promising approach for the repair of spinal cord injury.
Highlights
The mammalian central nervous system has been considered to remain generally unchanged during adulthood
Nerve cells were acquired from the tissue surrounding cervical spinal cord central canal and sorted by fluorescent-activated cell sorting (FACS) (Figure 1B)
The purity of the cerebrospinal fluid-contacting neurons (CSF-cNs) was approximately 99.34 ± 0.10%, as determined by immunofluorescence staining with the specific marker polycystic kidney disease type 2 channel 1 (PKD2L1) (Figures 1E,F)
Summary
The mammalian central nervous system has been considered to remain generally unchanged during adulthood. Recent research has confirmed that the mammalian central nervous system contains endogenous neural stem cells (NSCs) that exhibit self-renewal properties and are able to undergo multi-directional differentiation (Reynolds and Weiss, 1992). Research has shown that endogenous NSCs are located in specific niches within the dentate gyrus of the hippocampus and the central ependymal area of the spinal cord (Doetsch et al, 1999; Seaberg and van der Kooy, 2002; Hugnot and Franzen, 2011; Obernier et al, 2018), the exact composition of. The NSCs Properties of CSF-cNs these NSCs have yet to be determined This is because of the complex composition of cells in these areas, and due to the fact that cellular markers often overlap with each other
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