Abstract
Objective: To investigate the effect of microinjection of Nesfatin-1 into VMH on gastric motility in diabetic rats and its regulatory mechanism, and to study whether electrical stimulation of hippocampal CA1 region can regulate this process. Methods: The diabetic rat model was established, and the hippocampal-VMH nesfatin-1 pathway was observed by retrograde gold fluorescence tracing combined with fluorescence immunohistochemistry. The effects of microinjection of nesfatin-1 by VMH and electrical stimulation of hippocampus on gastric motility were observed in vivo. Results: Nesfatin-1 inhibited gastric motility in a dose-dependent manner, and astressin-B partially blocked the inhibition of nesfatin-1 on gastric motility; Nesfatin-1 immunoreactive neurons were present in the cytoplasm of hippocampal CA1 region and Nesfatin-1 was expressed in some fluorescent gold labeled cells; electrostimulation of hippocampal CA1 region could promote gastric motility in diabetic rats, and anti-NUCB2/Nesfatin-1 antibody could promote gastric motility in diabetic rats.Enhance the effect of electrical stimulation of hippocampal CA1 region on gastric motility. Conclusion: Nesfatin-1 injection into VMH can regulate gastric motility in diabetic rats, which may be related to the CRF system, and the hippocampal CA1 region participates in the regulation of gastric motility by nesfatin-1 in VMH.
Highlights
Diabetes mellitus is a metabolic disease with elevated blood sugar
Diabetic gastroparesis is a common complication of diabetes mellitus, and the incidence is increasing year by year
Nesfatin-1 was first found in the hypothalamus of rats by O-I et al Later studies showed that Nesfatin-1 could play a variety of roles in the central and peripheral regions.Nesfatin-1 is widely distributed in feeding-related brain regions, such as paraventricular nucleus (PVN), arcuate nucleus (ARC), ventromedial nucleus (VMH), hippocampus (Hi), amygdala, septal nucleus, brainstem, dorsal vagal nucleus, solitary tract nucleus and posterior region[2]
Summary
Diabetes mellitus is a metabolic disease with elevated blood sugar. Without standard treatment, long-term hyperglycemia can lead to various complications. Nesfatin-1 was first found in the hypothalamus of rats by O-I et al Later studies showed that Nesfatin-1 could play a variety of roles in the central and peripheral regions.Nesfatin-1 is widely distributed in feeding-related brain regions, such as paraventricular nucleus (PVN), arcuate nucleus (ARC), ventromedial nucleus (VMH), hippocampus (Hi), amygdala, septal nucleus, brainstem, dorsal vagal nucleus, solitary tract nucleus and posterior region[2]. Hippocampus is a part of the marginal nervous system It is mainly involved in the functional regulation of learning and memory. There are extensive fibrous connections between hippocampus and hypothalamus, brainstem and other regional centers It jointly regulates visceral functional activities, known as "visceral brain"[6]. CA1 region, explored the effect of microinjection of Nesfatin-1 into VMH on gastric motility in diabetic rats and its regulatory mechanism, and studied whether electrical stimulation of hippocampal CA1 region could regulate this process
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