Abstract

Plasmalemma vesicles were isolated in a sucrose-containing medium from wheat and oat roots and the net negative surface charge density was determined with 9-aminoacridine fluorescence [Chow, W.S. and Barber, J. (1980) J. Biochem. Biophys. Methods 3, 173-185]. The outer surface of the vesicles (measured in the presence of sucrose) had densities of − 16 to − 20 mC·m −2 and −29 mC·m −2 for wheat and oat roots, respectively. The inner surface - presumed to be the cytoplasmic side and calculated from the values measured in the presence and absence of sucrose - was more negative, and its size depended on the salt status of the roots.

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