Abstract
1,3-Propanediol (1,3-PD) is a valuable chemical intermediate in the synthesis of polyesters, polyethers, and polyurethanes, which have applications in various products such as cloth, bottles, films, tarpaulins, canoes, foam seals, high-resilience foam seating, and surface coatings. Klebsiella pneumoniae can produce 1,3-PD from glycerol. In this study, KPN00353, an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), was found to play a negative regulatory role in 1,3-PD production under microaerobic conditions via binding to glycerol kinase (GlpK). The primary sequence of KPN00353 is similar to those of the fructose-mannitol EIIA (EIIFru and EIIAMtl) family. The interaction between KPN00353 and GlpK resulted in inhibition of the synthesis of glycerol-3-phosphate (G3P) and correlated with reductions in glycerol uptake and the production of 1,3-PD. Based on structure modeling, we conclude that residue H65 of KPN00353 plays an important role in the interaction with GlpK. We mutated this histidine residue to aspartate, glutamate, arginine and glutamine to assess the effects of each KPN00353 variant on the interaction with GlpK, on the synthesis of G3P and on the production of 1,3-PD. Our results illuminate the role of KPN00353 in 1,3-PD production by K. pneumoniae under microaerobic conditions.
Highlights
Glycerol is a structural component of many lipids in organisms, and yeast can produce glycerol by the fermentation of sugar
This study demonstrates the role of KPN00353, an open reading frame (ORF) common to several K. pneumoniae isolates from different sources, in binding to GlpK, glycerol uptake and 1,3-PD production under microaerobic conditions and provides structural insight into KPN00353-GlpK interaction
To see whether the ORFs KPN00353-KPN00352-KPN00351 are specific to K. pneumoniae MGH 78578 or common to most K. pneumoniae isolates, we randomly selected eight K. pneumoniae strains from the NCBI database and compared their genomes with that of K. pneumoniae MGH 78578
Summary
Glycerol is a structural component of many lipids (glycerolipids) in organisms, and yeast can produce glycerol by the fermentation of sugar. 1,3-PD is a product of glycerol fermentation by Klebsiella, Enterobacter, Citrobacter, Lactobacillus, and Clostridium species and by engineered Escherichia coli (Huang et al, 2002; Saxena et al, 2009; Pyne et al, 2016). Among these organisms, Clostridium butyricum and Klebsiella pneumoniae are considered the best natural 1,3-PD producers (Saxena et al, 2009). The amount of 1,3-PD produced by K. pneumoniae was increased by the overexpression of 1,3-propanediol oxidoreductase (PDOR) or both PDOR and DhaB under microaerobic conditions (Horng et al, 2010)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
