Abstract
Spermiogenesis in mammals has been amply studied using conventional methods for electron microscopy (1). The ultrastructure of the neck region of spermatids has been analysed in some species (2) and appears formed by three main structures: the proximal centriole(PC), the distal centriole (DC) and the centriolar adjunct (CA) which is an extension of the PC composed by atypical triplets. All these microtubular (MT) structures have been specially analysed by means of conventional electron microscopy and only few analyses have been carried out using other methods, as cytochemical procedures, cell dissociation techniques,etc. For this reason in this communication the observations made on the neck structures after the application of cytochemical and dissociation procedures are reported. Moreover the observation of dissociated neck structures in spread and whole mount preparations has permited to apply the “in vitro” assembly of MT using purified MT proteins, thus testing the capacity of such structures to act as microtubule organizing centers (MTOC).
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