Abstract
The influence of strong continuous wave illumination on the yield of chlorophyll fluorescence was investigated using a two-beam method. This method is based on subnanosecond pulses to excite fluorescence and strong continuous wave light to induce variations of fluorescence yield. The application of a boxcar integrator (gate duration, 0.22 ns) enables us to measure the light-induced variations of fluorescence yield at different times during the fluorescence pulse and, thus, to investigate the relation of fluorescence induction to the different components of the multiexponential fluorescence decay. The experiments were carried out at room temperature using leaves of maize and spruce. We have determined the decay of continuous wave light-induced variable fluorescence to be single exponential with a lifetime of 2.0 ± 0.2 ns. The variable fluorescence is attributed to the recombination of separated charges in the reaction center of Photosystem-II.
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