The nano mixture of ethanol extract ketumbar (Coriandrum sativum L.) seed and putri malu (Mimosa pudica L.) leaves potentially reduced blood glucose levels in hyperglycemia wistar rat

Abstract

The formation of nano extracts can increase the bioactivity of a compound in the body. The purpose of this study was to determine the ability of a nano mixture of Coriandrum sativum L seed and ethanol extract of Mimosa pudica L leaves to reduce glucose levels in hyperglycemic rats, compared to an ethanolic extract mixture of Coriandrum sativum L seeds and Mimosa pudica L leaves. The size and zeta potential characterization of the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves was carried out using a Particle Size Analyzer (PSA). The results of characterization with PSA showed that the nanoparticle size of the ethanolic mixture of Coriandrum sativum L seeds and Mimosa pudica L leaves was 184.4 nm and the zeta potential value was -1.62 mV. The particle size obtained is in accordance with the herbal nano size, which is 50-300 nm. Identification of compounds in the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves by LC-MS/MS, found 7 compounds suspected of antihyperglycemia, namely phenylalanine, indole, 4-methoxy benzaldehyde, 2,5-dimethoxy benzaldehyde, apigenin, 2-flouren-9-ylidenemethyl-pyridine, and 5-Pentyl-1,3-benzenediol. The activity test results showed that the nano ethanol extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves was more effective in lowering blood glucose levels (79.29%) compared to the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves (50.88%).