The NADPH-diaphorase-containing system in the brain of the budgerigar (Melopsittacus undulatus).

Abstract

In the present investigation we studied the presence and distribution of histochemically detected neuronal NADPH-diaphorase (ND) in the brain of the budgerigar, Melopsittacus undulatus. Positive neurons are widely distributed throughout the central nervous system. ND-containing neurons are present in the telencephalon and the paleostriatal-parolfactory lobe complex. Positive cells were observed also in the neostriatum, including the main auditory area (field L), in several nuclei of the archistriatum and in the hyperstriatum (accessory, dorsal, and ventral). In the diencephalon, positive neurons were present both in the lateral hypothalamic and periventricular areas, and in a segregate area at the confluence of the anterior commissure and the lateral prosencephalic bundle. A group of positive perikarya was located lateral to the dorsal part of the IIIrd ventricle, and continued laterally into the thalamus. Weakly stained neurons were observed in the thalamic dorsomedial posterior nucleus. In the mesencephalon, ND-containing neurons were scattered in the reticular formation (pars lateralis and pars medialis) and in the optic tecta. A large population of positive neurons was observed in the substantia nigra, the ventral area of Tsai and the nucleus interpeduncularis. Positive neurons extended through the tegmental nuclei to the locus coeruleus. In the cerebellum, the granular neurons were weakly stained and the internal cerebellar nuclei were surrounded by a wide network of positive fibers. In the medulla the number of positive cells was highly reduced, but stained neurons were observed in the cochlear as well in the vestibular nuclei. The data here presented suggest that the distribution of ND-containing neurons in the brain of the budgerigar is different from those of the chicken and quail. The locations of positive neurons suggest also a possible involvement in sound perception and production pathways, and visual perception.