Abstract
The complete amino acid sequence of lysozyme Ch has been established by a combination of automated and manual Edman degradation and carboxypeptidase digestion.(see article)There is a single disulfide bond in the center of the molecule. The enzyme has 211 residues with a calculated molecular weight of 22,415. Lysozyme Ch has an amino acid sequence that is totally different from all other lysozymes whose sequences are known.
Highlights
Both the cyanogen bromide peptides [13] and the tryptic peptides [14] of lysozyme Ch have been separated and ordered
The complete amino acid sequence of lysozyme Ch is shown in Fig. 1, and the data required to document this sequence are given
Automated Edman Degradation-The majority of the sequence of lysozyme Ch was established by automated Edman degradation
Summary
From the Departments of Microbiology and Biochemistry, Vanderbilt University Tennessee37232. The sequences of several other lysozymes are known: guinea-hen egg white [4], turkey egg white [5], duck egg white [6], Japanese quail egg white [7], T2 phage [8], T4 phage [9], baboon milk [10] and human [11, 12]. Both the cyanogen bromide peptides [13] and the tryptic peptides [14] of lysozyme Ch have been separated and ordered. These peptides have been sequenced and the complete amino acid sequence of lysozyme Ch has been established
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