Abstract
We have analyzed the mutagenic effect of elevated temperatures (hyperthermia: HT) either upon direct exposure or after a previous heat shock (HS) in the haploid auxotrophic Saccharomyces cerevisiae strain SC7K lys2–3 in the logarithmic phase of growth. We demonstrated a significant antimutagenic effect of HS (38°C for 1 h) followed by hyperthermia (48°C, up to 60 min). Using cycloheximide (CHM) during the HS exposure we reversed the antimutagenic effect. We suggest that, upon HS, the enzymes involved in repair of premutational damage are more resistant to denaturation, i.e., by the induced HSP genes, and could work efficiently to prevent damage fixation in critical targets. CHM blocks the antimutagenic effect of the HS treatment by inhibiting the inducible synthesis of HSP.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
