The murine Mov-34 gene: Full-length cDNA and genomic organization

Abstract

The Mov-34 mutation is a recessive embryonic lethal mutation caused by experimental introduction of a recombinant Moloney murine leukemia provirus into the mouse germline. We have cloned a full-length cDNA from the Mov-34 gene, the transcription unit disrupted by the proviral integration. This cDNA is predicted to encode a novel 321-amino-acid, 36-kDa protein of unknown function. Overlapping phage λ clones containing the entire Mov-34 gene have been isolated. The Mov-34 gene spans just over 8 kb and contains seven exons. The 5′ flanking region of the Mov-34 gene contains neither “TATA” nor “CAAT” box sequences, and 5′ end mapping by primer extension and ribonuclease protection reveal multiple transcription initiation sites.