Abstract
Determining the phage susceptibility of staphylococci is complicated by the appearance of “inhibition,” in which concentrated phage suspensions produce confluent lysis, but no dilution of the phage suspension produces distinct plaques. Variants of typing phage 81 were found to inhibit cultures A17 and A19. The multiplication of phage in these nonpermissive hosts was studied by using more sensitive indicator strains for phage assays in one-step growth experiments. The phage produced was neither host-modified nor mutant. Since there have been reports of endemic hospital infections due to untypable strains inhibited by group III phages, two such strains, CL-4 and CL-63, were studied for production of typing phages 47 and 53. It was found that inhibition may be associated with either a normal burst of phage from a small fraction of infectious bacterial centers or with the release of a reduced burst from a higher proportion of infectious centers. The multiplication of phage in some cells of these nonpermissive hosts appeared to depend upon the physiological state of the culture as well as the genetic potential of the phage.
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