Abstract
For the purpose of obtaining the high-titer suspension of poliomyelitis virus, the author cultivated the virus on monkey kidney cells and human amnion cells in vitro using the sub-synthetic mixture including casaminoacid, which was simplified synthetic mixture No. 199 in our laboratory. This subsynthetic mixture could be used sufficiently for the cultivation of the polio virus as well as the mixture No. 199. At the same time, the author discribed the process of the cytopathogenic effect observed in monkey kidney cells and human amnion cells infected with polio virus in vitro. The author observed the appearance of eosinophilic masses in the cytoplasm and the nuclear pyknosis. And finally the infected cells showed the cytolysis accompanied with cytoplasmic bubbling.
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