The multiplex polymerase chain reaction for detection of ventilator-associated trachea-bronchitis and pneumonia in patients with common pathogens

Abstract

To explore the clinical utility of multiple polymerase chain reaction (M-PCR) in the rapid detection of the common pathogens in ventilator-associated trachea - bronchitis (VAT) and ventilator-associated pneumonia (VAP). Sputum samples of 75 patients complicated VAT or VAP in surgical intensive care unit (SICU), were examined by bacterial culture, ordinary PCR, the M-PCR detection. The pathogen detection rates among three methods were compared. The Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae of the positive detection rates were 50.7%, 45.3%, 30.7%, 41.3% and 58.7% by bacterial culture. By ordinary PCR, the positive detection rates were respectively 88.0%, 89.3%, 78.7%, 85.3% and 93.3%, and by M-PCR, the positive detection rates were respectively 92.1%, 90.7%, 82.7%, 89.3% and 96.0%. The positive rates of five common pathogens of ordinary PCR and M-PCR were higher than those of bacterial culture (all P < 0.05). The M-PCR had merit for rapid detection compared with ordinary PCR. Compared with bacterial culture, ordinary PCR and M-PCR yield higher positive rates in identifying five common pathogens of VAT and VAP, meanwhile, it also demonstrated the tendency that M-PCR may save cost and labor power.