Abstract
A method for the separation and analysis of conjugates of neutral steroids in urine is described. Following Amberlite XAD-2 extraction of steroids, separation into neutral, glucuronide, monosulphate and disulphate conjugate groups was achieved by ion exchange chromatography on the lipophilic gel, diethylaminohydroxypropyl Sephadex LH-20 (DEAP-LH-20). Enzymatic and solvolytic procedures were used to hydrolyse the conjugate moiety and the steroids were analysed by gas-chromatography using open-tubular glass capillary columns after the preparation of the O-methyloxime-trimethylsilyl ether derivative. Characterization of the steroids was made by computerised GC-MS following repetitive magnetic scanning? Examples of the application of the method to the analysis of urine from normal male subjects, and a patient with Cushing's syndrome are presented.
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