Abstract
Smooth muscle gamma actin (Actg) is expressed in smooth muscle and in haploid male germ cells. In order to further characterize the Actg gene, a 60-nucleotide-long isotype-specific probe was synthesized. Single bands of DNA were detected when this oligonucleotide was used to probe blots of mouse genomic DNA digested with PstI, EcoRI, KpnI, or XbaI. These results suggest Actg is a single-copy gene with no detectable pseudogenes. The Actg gene was mapped to mouse chromosome 6 by Southern blot analysis of DNA isolated from 15 mouse-hamster hybrid cell lines.
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