The mouse model of glaucoma filtration surgery: from understanding the wound healing response to applications as a tool for validating anti‐fibrotics

Abstract

AbstractPurpose: Failure after glaucoma filtration surgery (GFS) is attributed to fibrosis at the operated site. We have developed a mouse model for GFS so as to understand the wound healing process after this procedure and to test anti‐fibrotics for future clinical application. Methods: Modified GFS was performed on mice and bleb survival was determined by slit lamp photography. Real‐time PCR, antibody‐based cytokine assays, flow cytometry, and immunofluorescent analyses were performed to determine gene and cellular changes. Results: The mouse model of GFS mimicked the clinical response to mitomycin C. Further analysis of the operated tissues revealed that the post‐surgical wound healing response was separable into two distinguishable phases: an early “acute inflammatory” phase followed by a late “fibrotic” phase. Conclusions: Our data suggest that targeting the distinct phases of the wound healing process after GFS may be desirable in order to overcome the long term scarring response. Furthermore, the mechanisms of potential anti‐fibrotics may now be understood in terms of their specific effects on each phase of the wound healing process. Paper presentation at Anatomy & Cell Biology, Special Interest Symposium.