The morphology of staphylococcal bacteriophage K and DNA metabolism in infected Staphylococcus aureus.

Abstract

The morphology and dimensions of bacteriophage K particles were determined by electron microscopy. This virus had an icosahedral head (approx. 70 nm diam.) and a long (210 nm) thin (15 nm) contractile tail which terminated in a complex basal appendage. The precise dimensions of the particles were dependent on the negative stain employed. The buoyant densities of the K virus particle and its DNA were 1 X 479 g/ml and 1 X 689 g/ml respectively. The DNA had a base composition of 30% G + C, a contour length of 16 X 1 micron and a calculated mol. wt. of 33 X 10(6). With Staphylococcus aureus (NCTC 9318) as host, the latent period was 25 min, the eclipse period 14 min and the average burst size 60 p.f.u./bacterium. Infection resulted in inhibition of host DNA synthesis and degradation of the bacterial DNA: the products were used for tee synthesis of phage DNA. The kinetics of DNA synthesis is infected and uninfected bacteria were examined. There was no initial cessation of DNA synthesis in the infected bacteria.