THE MORPHOLOGICAL EVALUATION OF THE EXPERIMENTAL MODEL OF SYSTEMIC GENERALIZED THROMBOSIS

Abstract

The only regulated model of disseminated intravascular coagulation syndrome or disseminated intravascular coagulation syndrome is the Di Minno model of generalized thrombosis. Due to the expansion of the role of tissue factor and thrombin in the initiation of DIC, the aim of this study is the histological assessment of the Di Minno model under conditions of DIC initiation by tissue factor or thrombin with comparison with the classical variant. The study was performed on 80 male mice. All laboratory animals were divided into three groups depending on the trigger of thrombosis: Group I - collagen + adrenaline solution (0.5 mg / kg + 0.06 mg / kg), Group II - 10 mg / kg thromboplastin, Group III - 10 mg / kg thrombin. Histological studies of animal’s preparations demonstrate a lower content of blood clots in the lungs of laboratory animals, which suggests that the main cause of death of animals is not acute thrombosis, but the development of delayed manifestations of DIC when using tissue factor. In the case of thrombin using, on the contrary, the death of animals is recorded on the first day of the experiment and the results of the histological study demonstrate the massiveness of thrombosis, which is not initially provided for by this model, calculated to assess the effectiveness of new antiplatelet drugs in an acute experiment. Thus, on the basis of morphological studies, the limitations of the use of tissue factor and thrombin in model thrombosis have been demonstrated and the expediency of using physiological inducers of aggregation has been substantiated.