THE MORPHOLOGICAL CHARACTERISTICS OF THE OFFSPRING OF MALE RATS IRRADIATED WITH ELECTRONS AT A DOSE OF 2 GY AND TREATMENT BY PLATELET-RICH PLASMA

Abstract

One of the most dangerous effects of radiation on health is reproductive dysfunction, often leading to infertility. Available studies on the effects of radiation on spermatogenesis and fertility have not considered the possibility of using restorative therapy. One of the modern methods of infertility treatment is the introduction of autologous blood plasma with an increased content of platelets and native growth factors. Currently, there are no studies on the study of offspring obtained by using this method of infertility treatment and the assessment of its teratogenicity. Purpose of the study: morphological analysis of offspring of male rats irradiated with electrons at a dose of 2 Gy and receiving platelet-rich plasma. Male Wistar rats (n=35) were once subjected to targeted ionizing radiation at a NOVAC-11 pulsed electron accelerator at a dose of 2 Gy. To half (n=15) of the irradiated animals were injected with platelet-rich plasma and insulin-like growth factor-1 for 12 weeks, after which the males of both groups were placed with intact females (n=30) to produce offspring. The state of the developing offspring was assessed by morphological analysis after the removal of pregnant females from the experiment on the 20th day of prenatal development. Pregnancy was observed only in females bred by control males and males treated with platelet-rich plasma. Classical histological methods and X-ray computed microtomography were used. As a result of the study, it was found that the weight and size of the offspring from the control and experimental groups did not have macroscopic differences; light microscopy did not reveal significant changes in their histological structure. Pathological changes in the development of the offspring of males of the experimental group who received platelet-rich plasma after electron irradiation with a dose of 2 Gy using computed microtomography were also not detected. The obtained data indicate about of effectiveness of the treatment of the infertility of irradiated male rats with platelet-rich plasma, as well as the absence of its teratogenic effects.