Abstract
Longidorus piceicola, a new geographical and host record from Romania, was described and illustrated on the basis of two populations originating from a coniferous and a deciduous forest. The main morphological characters of specimens from Romania correspond very well with the type material collected from the soil around Picea abies L. (Slovakia) except for the shorter body and tail. The D2-D3 fragment of 28S rDNA from both populations was amplified and sequenced, and the sequences were identical to L. piceicola sequence from Slovakia. The partial 18S-ITS1-5.8S-ITS2 rDNA regions from one of the populations were sequenced for the first time. The evolutionary relationships between L. piceicola and the closest species L. intermedius based on D2-D3 sequence divergence and single-nucleotide polymorphisms are discussed. Although having very low sequence dissimilarity (0.3–0.9 %) both species have distinct morphology and biology. Longidorus piceicola differs from L. intermedius in having a much longer odontostyle, body, distance anterior end - guide ring, a wider lip region, more ventromedian supplements (11 vs 5–7) in the male, and develops through four rather than three juvenile stages. Furthermore, L. piceicola occurs more frequently in association with conifers, while L. intermedius is found mainly in oak forests.
Highlights
Longidorus piceicola Lišková, Robbins & Brown, 1997 was originally described from Slovakia (Lišková et al 1997) in association with Picea abies L. It was recovered from different localities in Bosnia and Herzegovina, Serbia and Montenegro (Barsi and Lamberti 2001), and Poland (Kornobis and Peneva 2011) in forests dominated by coniferous trees
Specimens were collected from the rhizosphere of a Larix decidua Mill. forest near to Bran, Braşov County, Romania (45.3050N, 25.2156E), ca 760 m a.s.l. on 15.10.2013, and from the soil around roots of deciduous trees (Quercus sp., Tilia sp., and Fraxinus sp.), Cernica forest, Ilfov County (44.2637N, 26.16514E) and ca 60 m a.s.l. on 4.08.2014
Eleven females and 21 juveniles, two females and one juvenile from Cernica forest, Ilfov County, Romania on slide numbers NE 35–37 stored at the reference collection of the National Phytosanitary Laboratory, Voluntari, Romania, 9 females and 20 juveniles - at the personal collection of the first author; nine females and 30 juveniles from Bran, Braşov County, Romania, stored in the nematode collection of IBER, Bulgaria, slide numbers N2-29/2/1-19
Summary
Longidorus piceicola Lišková, Robbins & Brown, 1997 was originally described from Slovakia (Lišková et al 1997) in association with Picea abies L. It was recovered from different localities in Bosnia and Herzegovina, Serbia and Montenegro (Barsi and Lamberti 2001), and Poland (Kornobis and Peneva 2011) in forests dominated by coniferous trees. Two new findings of this species in Romania are reported. The aims of this paper are to characterize morphologically and molecularly the populations recovered and to discuss the phylogenetic relationships with the most closely related species
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