Abstract
In the agricultural practice one ram is used for the insemination a lot of ewes according to the breeding plan. For the insurance of the successful insemination of ewes reached the heat simultaneously, it is necessary to obtain some consistently ejaculates from one ram. The aim of the present work was the estimation of the sperm quality in consecutive ejaculates from Il-de-France rams at various ages. It was investigated over 40 ejaculates from 5 rams of the IAS-Kostinbrod herd between 1, 5 and 4 years old during the breeding season. The sperm was collected by artificial vagina. It was estimated the following morphological and biochemical parameters: volume, count of the spermatozoids in 1ml, motility, survivability at 39?C and activity of the alkaline phosphatase in the semen plasma. In the consecutive ejaculates of both groups no significant morphological changes of spermatozoids were established. The obtained results are shown that the concentration of the spermatozoids and the activity of the alkaline phosphatase in the semen plasma reduce in the third consistently ejaculate, but the keeping of Aph activity in second ejaculates despite decreasing of the sperm volume and concentration of spermatozoid. On the base of these results we could give reference for use of the first and second ejaculates in the practical insemination of ewes.
Highlights
Introduction and literature reviewIt is known that reproductive potential of different males give possibility to obtain some ejaculates from one male per day
The main objective of our study was the estimation of the sperm quality in consecutive ejaculates from Il-de-France rams at various ages obtained during the breeding season
It was observed that the sperm volume and concentration of spermatozoids in mature rams was greater than in young in each consecutive ejaculate
Summary
Introduction and literature reviewIt is known that reproductive potential of different males give possibility to obtain some ejaculates from one male per day. The ability to collect two or more consecutive ejaculates in a short period could be valuable for gamete resource banking and preserving genetic diversity of the native breeds in different animal species. In this way, there are a former and present investigations aimed to estimate the quality of the consecutive ejaculates (Parker and Thwaites,1962; Mattner and Voglmayr ,1962; Ritar et all, 1992; Shamsuddin, et al, 2000; Nel-Themaat et al.,2006). How many ejaculates can be collected and if all of them have the good quality? It is impossibly to give exactly answer, because the literature data are very scanty
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