THE MORFOFUNCTIONAL STATE OF PERIPHERAL ERYTHROCYTES AT CHRONIC HEART FAILURE AND VITAMIN D DEFICIENCY IN

Abstract

The aim of the work To study the effect of vitamin D deciency in blood plasma on the morphofunctional state of peripheral blood erythrocytes after exercise in patients with chronic heart failure. Methods: scanning electron microscopy of peripheral blood erythrocytes, enzyme immunoassay to determine the level of vitamin D. Results. A morphological study revealed heterogeneity of erythrocyte subpopulations in 90% of patients with CHF and vitamin D deciency. In peripheral blood, in addition to normocytes with sufcient hemoglobinization, subpopulations of macro- and microcytes were recorded. In most patients with vitamin D deciency, several types of morphological changes in the shape of erythrocytes were revealed, manifested in the form of echino-, acantho- and stomatocytosis, which indicates destabilization of the erythrocyte's membrane. After a 6-minute walk test in CHF patients with vitamin D deciency, the number of discocytes decreases, the ability to deform decreases, lytic cells were recorded with a reduced number of normocytes. The Conclusion: identied morphobiochemical changes indicate the destructurization of the erythrocyte membrane components and have negative consequences, taking into account the likely impact on the somatic health of patients who, in addition to changes characteristic of CHF, receive signicant stress during physical exertion against the background of hypovitaminosis. D.