The monazomycin probe as detector of the interaction of charged lipid vesicles, polypeptides, proteins, and divalent cations with planar bilayer lipid membranes

Abstract

The antibiotic monazomycin, operated in a current-clamp mode, is shown to provide a convenient, sensitive, and continuous monitor of the interactions of charged moieties, including biological materials, with planar bilayer lipid membranes. The technique is quite general; its use is illustrated by demonstrating the interactions of phosphatidylserine vesicles, poly-L-lysine, cytochrome c, and MgSO 4 with planar lipid bilayers.