THE MOLECULAR STUDY OF LncRNA “NEAT2” FOR EARLY DIAGNOSIS OF TB IN POPULATION OF MADHYA PRADESH

Abstract

One of the deadliest infectious diseases in the world is tuberculosis (TB), a chronic disease brought on by the bacteria Mycobacterium tuberculosis. There is still no viable method for early screening, despite substantial advancements in tuberculosis detection methods and therapeutic strategies. Different cellular processes can hinder the growth of TB due to dysregulation of gene expression patterns connected to particular substances. Long non-coding RNAs (lncRNAs) are molecules that have been found to control the expression of protein-coding genes and take part in processes like cell cycle control, gene silencing, and cellular differentiation. It has been discovered that dysregulation of lncRNAs is linked to a variety of illnesses, including cancer and infectious diseases. Therefore, it is encouraging that lncRNAs have been identified as potential molecular indicators and therapeutic targets for tuberculosis. In present study. Human blood samples from the TB patients and healthy individuals from Sidhi District of Madhya Pradesh were collected. Total RNA were extracted from blood samples of using TRIzol Reagent (Takara) according to the manufacturer’s protocol. cDNA were prepared using synthesis kit (Biorad), In the present investigation gene expression of LncRNA “NEAT2” and IFNG were assessed in WBCs of TB Patients and Healthy individuals, using Real Time PCR technique. This study reported Lnc RNA NEAT2 up-regulation, which reflect significant involvement of this LncRNA in pathogenesis of TB and it may be one of the potential marker for the early diagnosis of TB.