Abstract
BackgroundFertilization in flowering plants depends on the early contact and acceptance of pollen grains by the receptive papilla cells of the stigma. Deciphering the specific transcriptomic response of both pollen and stigmatic cells during their interaction constitutes an important challenge to better our understanding of this cell recognition event.ResultsHere we describe a transcriptomic analysis based on single nucleotide polymorphisms (SNPs) present in two Arabidopsis thaliana accessions, one used as female and the other as male. This strategy allowed us to distinguish 80% of transcripts according to their parental origins. We also developed a tool which predicts male/female specific expression for genes without SNP. We report an unanticipated transcriptional activity triggered in stigma upon incompatible pollination and show that following compatible interaction, components of the pattern-triggered immunity (PTI) pathway are induced on the female side.ConclusionsOur work unveils the molecular signatures of compatible and incompatible pollinations both at the male and female side. We provide invaluable resource and tools to identify potential new molecular players involved in pollen-stigma interaction.
Highlights
Fertilization in flowering plants depends on the early contact and acceptance of pollen grains by the receptive papilla cells of the stigma
We report an unanticipated transcriptional activity triggered in stigma upon incompatible pollination
On the other hand, based on Gene Ontology enrichment study and pathway-based prediction of upregulated genes we show that following compatible pollination, components of the pattern-triggered immunity (PTI) pathway are induced on the female side
Summary
Fertilization in flowering plants depends on the early contact and acceptance of pollen grains by the receptive papilla cells of the stigma. The early interaction between the extremity of the female organ (stigma) and the male gametophyte (pollen grain) acts as a checkpoint for fertilization This first step of the female-male interaction includes recognition by the female tissues of the male partner. The stigmatic EXO70A1 protein was identified as a factor required for polarized secretion during compatible pollination, which is negatively regulated in incompatible reaction [11, 13]. While these features are well established, they constitute a narrow framework that limits the understanding of the whole recognition process.
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