The Molecular Phylogeny of Five Eastern North Pacific Octopus Species

Abstract

The DNA sequence of a 612 nucleotide fragment of the mitochondrial cytochrome oxidase subunit III gene from five Octopus species has been determined. The COIII gene in these species shows an extreme bias against G in the DNA sense strand with a moderately low C composition. The bias against G and C severely restricts the codon usage in Octopus COIII genes. The aligned DNA sequences were subjected to distance, maximum-likelihood, and parsimony analyses to ascertain the phylogenetic relationship of the species. The results of all of the analyses were concordant. The analyses indicate that O. bimaculoides and O. bimaculatus are the least diverged of the species and fall into a separate clade from O. dolfleini and O. californicus, which are also closely related. O. rubescens is about equally removed from the other species, but parsimony, distance, maximum-likelihood, and logdet analyses suggest that it is more closely aligned with the O. bimaculoides/ O. bimaculatus lineage.