Abstract
Zanthoxylum armatum DC., belonged to the family Ruteacea, is a medicinal plant used to cure many diseases. DNA barcoding was used as a tool for molecular identification of Zanthoxylum armatum DC. species from Balakot Pakistan. In the present study four DNA barcodes including matk, rbcl, ITS and trnH-psbA were used. The sequenced data were analyzed by using BLASTn at NCBI, FASTA and Mega 7.0 software. During PCR analysis, 3 DNA barcodes ITS, rbcl and trnh-psbA were successfully amplified and showed the 100% sequencing success. Furthermore, these barcode markers showed 99-100% sequence similarity with the reference sequences at the BLASTn. The further analysis revealed the sequence similarity of investigating marker with Zanthoxylum armatum (MH016484.1), Zanthoxylum nitidum (FN599471.1) and Zanthoxylum bungeanum (MF097123.1) respectively. The current finding provides the basis for sequenced data of Z. armatum to be used in future for molecular discrimination among the plant species from Pakistan and it is concluded that combination of diverse kind of barcoding markers could be helpful in proper identification of species at lower taxonomic level.
 Bangladesh J. Plant Taxon. 27(2): 323-333, 2020 (December)
Highlights
Plants were used by the peoples for their nourishing requirements
Various efforts have been made to validate the occurrence of several plastid and ribosomal markers and authenticated DNA barcodes have been reported from plant species of Pakistan (Khan et al 2019a,b)
The current study is the continuity of our previous efforts and here we are investigating that how combined markers could be effective in the molecular systematics and phylogenetic reconstruction of medicinally important plant species from Pakistan
Summary
Plants were used by the peoples for their nourishing requirements. With the passage of time, the natural flora has become an important source across various human communities for health improvement and remedies against several diseases. Various efforts have been made to validate the occurrence of several plastid and ribosomal markers and authenticated DNA barcodes have been reported from plant species of Pakistan (Khan et al 2019a,b). The current study is the continuity of our previous efforts and here we are investigating that how combined markers (ribosomal and plastid) could be effective in the molecular systematics and phylogenetic reconstruction of medicinally important plant species from Pakistan.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
