The Molecular Genetics of Self-incompatibility in Petunia hybrida

Abstract

Abstract The cultivated petunia ( Petunia hybrida ) has been a popular system in which to study genetic, physiological and biochemical aspects of gametophytic self-incompatibility. As with other members of the Solanaceae a number of S-RNase genes have been isolated for functional S -alleles. We have identified S-RNase sequences for two additional functional S -alleles, S v and S 3 . These alleles are more similar to alleles from other families of the Solanaceae ( Nicotiana and Solanum ) than to any petunia alleles reported previously. The total number of S -alleles in P. hybrida is at least ten in spite of its cultivated origin. However, most cultivars of P. hybrida are in fact self-compatible and this appears to arise from the prominence of a single previously described allele S o . The implications of this observation for the origin of self-compatibility in P. hybrida are discussed. The S -locus of P. hybrida has recently been mapped using an indirect method involving T-DNA insertions. Seven T-DNA insertions that were previously shown to be closely linked to the S -locus were physically mapped on the long arm of chromosome III using fluorescent in-situ hybridization. The most tightly linked T-DNA insertions are in a sub-centromeric position. This is consistent with the centric fragments of P. inflata obtained by irradiation mutagenesis that carry additional S -loci and confer a pollen-part mutant phenotype. An S -linked restriction fragment length polymorphism (RFLP) marker, CP100 was used to confirm this chromosomal assignment and has provided evidence for S -locus synteny in the Solanaceae.