The molecular characterization of antioxidant enzyme genes in Helicoverpa armigera adults and their involvement in response to ultraviolet-A stress

Abstract

Ultraviolet (UV) radiation can cause oxidative stress in insects. To gain insight into the roles of different antioxidant enzymes of Helicoverpa armigera adults in response to oxidative stress caused by UV-A at a molecular level, three antioxidant enzyme genes [encoding copper zinc superoxide dismutase (Cu/ZnSOD), catalase (CAT) and glutathione peroxidase (GPX)] were cloned. The deduced amino acid sequences of these genes are similar to that of other insects. In addition, the expression profiles of genes from a classic antioxidant defense system, comprising Cu/ZnSOD, manganese superoxide dismutase (MnSOD), CAT, thioredoxin peroxidase, GPX and glutathione-S-transferase, were also determined. The results indicated that all antioxidant enzyme genes (except MnSOD in females) were significantly upregulated at certain time points (from 30 to 90min) in both male and female adults of H. armigera following UV-A radiation. We also found that longer periods of radiation exposure did not yield higher levels of mRNA expression. Furthermore, changes of determined physiological parameters (glutathione, Cu/ZnSOD and MnSOD activity) were basically consistent with the gene expression profiles. We therefore infer that the different antioxidant enzymes of H. armigera act in a coordinated manner at the transcriptional level against oxidative stress caused by UV-A radiation.