The molecular characteristics of soybean ARR-B transcription factors

He Li,Jiaxin Lin,Fengqiong Chen,Zhongcheng Chen,Xijun Jin,Chunyuan Ren,Qiusen Chen,Yuxian Zhang,Gaobo Yu,Runan Chen

doi:10.32604/biocell.2022.018762

Abstract

The Type-B authentic response regulator (ARR-Bs) gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes. However, study of ARR-Bs gene family in soybean is limited. Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome. 31 ARR-Bs genes (namely GmARR-B1-31) were identified, containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids (aa) and 28.30 to 76.86 kDa, respectively. Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I, Cluster II, and Cluster III—which included 15, 12, and 4 genes, respectively, and were asymmetrically distributed on 17 chromosomes. Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers, roots and seeds. The subcellular localization of GmARR5, GmARR14 were observed in the nucleus, and the promoter region of them included low-temperature responsive element (LTR), the C-repeat/dehydration responsive element (DRE), MBS, ABRE, MYB, MEJA and TCA-elements, which possibly participate in abiotic stress and hormones responses. qRT-PCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses, especially cold stress and salt stress, and GmARR-B5 and GmARR-B14 were significantly induced by cold stress. This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.

Highlights

Soybean (Glycine max (L.) Merrill) is one of the significant cash crops worldwide
The subcellular localization of GmARR5, GmARR14 were observed in the nucleus, and the promoter region of them included low-temperature responsive element (LTR), the C-repeat/dehydration responsive element (DRE), MBS, ABRE, MYB, MEJA and TCA-elements, which possibly participate in abiotic stress and hormones responses. qRTPCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses, especially cold stress and salt stress, and GmARR-B5 and GmARR-B14 were significantly induced by cold stress
For the nomenclature of each putative gene, the prefix “Gm” (for Glycine max (L.) Merrill) was added to ARR followed by type “B” and Arabic numbers. 31 type-B GmARR genes were named from GmARR-B 1 to GmARR-B 31 according to their physical locations on chromosomes 1–20

Summary

Introduction

Soybean (Glycine max (L.) Merrill) is one of the significant cash crops worldwide It can be an excellent source of protein, oil, and specialized metabolites such as isoflavonoids and saponins, and improve soil by microbial nitrogen fixation (Abdelghany et al, 2020; Hina et al, 2018). Transcription factors are molecular switches that control gene expression, so they play an important role in plant response to abiotic stress (Kidokoro et al, 2014). It has been demonstrated that the type-B authentic response regulators (type-B ARRs) gene family is one of the large plant-specific transcription factors family, which play an essential roles in response to abiotic stresses (D’Agostino, 2000). The C-terminal region is highly variable, but they all have a small conserved motif

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Conclusion