The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.

Abstract

The complex molecular biologic study of ticks, took off from people in the Omskii oblast during Spring-Summer periods in 2014-2016 established their infection with agents of tick's transmissive infections and testifies a significant risk of infection of population. The analysis of ticks I. persulcatus established that rate of detection of DNA of borrelia made up to 59,5%, rickettsia - 30.6%, anaplasmae and erlichiae - 5.9%. The analysis of ticks D. reticulatus by polymerase chain reaction detected DNA of rickettsia in 20.5%, borrelia - in 6.6%, RNA of tick-borne encephalitis virus - in 3.6%. No DNA of anaplasmae and erlichiae was detected. During sequencing of tests positive to rickettsia the most often Rickettsia raoultii was identified. The approaches to expressdiagnostic and preventive therapy of tick-born transmissive infections were substantiated with consideration for mixt-infection of agents and pathogenic characteristics of developing nosologies. The algorithms of laboratory analysis regulated by the Sanitary Regulations 3.1.33-10-15 "Prevention of infections transmitted by ticks" require a broader implementation.