The molecular and morphological characterization of the polyclonal antibodies isolated from poultry eggs.

Abstract

The humor immune response is mediated by antibodies synthesized as specific response against antigens. Our investigation aimed to emphasize the presence of the natural IgY antibodies from hen, quail, and duck eggs, and their molecular and morphological characterization. After the electrophoresis of the IgY antibody, three bands of samples from the hen, quail, and duck eggs were obtained. They correspond to heavy (H) and light (L) chains and trunk forms (? Fc), respectively. In ducks, the high frequency of the heavy trunk chains was recorded. The presence of the intermediary bands can be also observed, indicating the presence of the impurities in the immunoglobulin isolated from duck eggs in higher quantities compared to those recorded in hen and quail eggs. In the electron microscopy study of immunoglobulin, and from the images analyzed we can identify singular forms witch were morphological characterized. First, we observed the ”Y” antibodies forms but also the morphological aspects. From a detailed view of the images from this three species, we can observe particular aspects in the duck case. Electron microscopy images relieve great form similarity between quail and hen antibodies, and particular aspects in the duck case, where we can observe antibodies without terminal domains.