Abstract
Objective To explore the modulation and mechanism of Apelin-13 in an acute pain model. Methods The pain thresholds were continuously monitored in 60 min after intracerebroventricular (ICV) administration of Apelin-13 (0.05, 0.1 and 0.5 μg/g) and casein kinase 2(CK2) inhibitor 4, 5, 6, 7-tetrabromoben: zotriazole (TBB) by radiant heat tail-flick test.Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot. Results ICV injection of Apelin-13 induced a dose and time-dependent antinociceptive effect.This effect was signicantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480, which decreased obviously by TBB. Conclusion Apelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480. Key words: Apelin; Acute pain; Analgesia; Casein kinase Ⅱ; NR2B
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