Abstract
The mode of uptake of mitomycin C (MMC) was examined by use of rat ascites hepatoma AH130 cells. The uptake of MMC into AH130 cells increased in proportion to the cell number and the extracellular concentration of MMC. The uptake was nonsaturable and temperature-dependent but not inhibited by metabolic inhibitors such as ouabain, 2, 4-dinitrophenol, sodium azide or iodoacetic acid. MMC was rapidly metabolized in the cells and its metabolites accumulated with the time of incubation. Intact MMC in the cells was only a little. The uptake of MMC was, however, not affected by the metabolites accmulated in the cells. Amphotericin B potentiated the uptake and cytotoxicity of MMC. On the other hand, dibucaine diminished not only the uptake but also the cytotoxicity of MMC.These results suggest that MMC is taken up into rat ascites hepatoma AH130 cells in an energy-independent manner but the uptake is non-saturable because MMC is rapidly metabolized in the cells.
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