Abstract
Involution of pre-existing carrageenin granuloma in rats was brought about by local injection of hydrocortisone acetate(HC). In in vitro incubation of minced granuloma removed from rats injected locally with HC 6 hr prior to sacrifice, the release of perchloric acid soluble tyrosine (Tyr) into the medium was significantly elevated, while non-steroidal anti-inflammatory drugs had no effect on the release of Tyr. The increase of degradation, in minced granuloma exposed in vivo to HC for 6 hr, of 3H-Tyr-IabeIed tissue protein was suggested with a similar method as the incubation of non-labeled granuloma. Both in vivo and in vitro incorporation of 3H-Tyr into tissue protein of the granuloma was reduced by HC administered in vivo 6 hr before. Thus, both the enhancement of catabolism and the reduction of anabolism were manifested as the effect of HC on the granuloma. The predominance of anti-anabolic effect was deduced from the result of incubation experiments in which puromycin or cycloheximide was used in vitro with or without HC-treatment.
Published Version
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