Abstract
Hec1 (highly expressed in cancer) is a member of a conserved Ndc80 (nuclear division cycle 80) complex that regulates mitotic processes. Its overexpression is seen in various tumours and is associated with cancer progression. However, its expression pattern and role inhuman prostate cancer (PCa) still not clear. The aim of our study is to investigate the expression and functional role of Hec1in human PCa. Hec1 expression was measured in 10 pairs of PCa cancerous and non-cancerous tissue samples by quantitative real-time (qRT)-PCR. The effects of Hec1 on PCa cells were studied by RNAi approach. Apoptosis and cell cycle were analysed by flow cytometry. Cells viability was evaluated using cell counting Kit-8. Cyclin B1-Cdc2 (cell division cycle 2) activity was measured by ELISA assay. Long non-coding (Lnc)RNAs regulated by Hec1 were gained from bioinformatics analysis. The role of LncRNA BX647187, regulated by Hec1, was finally characterized in PCa cells by siRNA. Our results showed that Hec1 mRNA and protein were significantly overexpressed in Human PCa tissues and several PCa cell lines. Silencing Hec1 markedly suppressed proliferation, promoted apoptosis and induced cell-cycle arrest in G2/M-phase in PCa cells. Through bioinformatics analysis and knockdown Hec1in PCa cells, we found LncRNA BX647187 was positively regulated by Hec1. We further demonstrated that suppression of BX647187in PCa cells significantly reduced cell proliferation and promoted apoptosis. Thus, we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187. Our studies may contribute to understand the molecular mechanism of PCa pathogenesis and clinical therapy.
Highlights
Prostate cancer (PCa) is the most common malignancy and a common cause of cancer-related mortality for men all over the word
highly expressed in cancer (Hec1) was highly expressed in prostate cancer tissues and cell lines To evaluate the functional role of Hec1 in human PCa, the expression levels of Hec1 in 10 pairs of PCa tissue and adjacent non-tumour samples were determined by quantitative real-time (qRT)-PCR assays
We report two novel findings: (i) Hec1 is consistently overexpressed in human PCa and cell lines and (ii) Hec1 is closely linked with human PCa development and progression through the meditator Long non-coding RNA (LncRNA) BX647187 in vitro
Summary
Prostate cancer (PCa) is the most common malignancy and a common cause of cancer-related mortality for men all over the word. PCa affects more than 240000 men in the U.S.A. and increasing numbers of new cases are diagnosed each year [1]. PCa is recognized as a major and increasing health problem in China. Despite the considerable advances in diagnosis and adjuvant therapy, the clinical outcome of PCa patients has not been improved markedly. Pathogenesis, diagnosis and medical treatments of PCa still are substantial clinical challenges. There is an urgent necessity to improve the molecular characterization of PCa, in order to facilitate understanding of PCa pathogenesis and improvement on diagnostic and therapeutic efficiency
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