Year-end Sale % OFF 
Abstract
The mitotic index of the roots of pigeon pea can be the basis for determining the growth of pigeon pea. The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells. The preparation of the pigeon pea was carried out for 4 days to grow the roots. The roots were cut off at 08.00, 08.15, and 08.30 WIT (Eastern Indonesian Time). The roots were cut 0.5-1cm. Carnoy’s solution was used as the fixative solution using the Squash technique. The prepared roots were then observed using an Olympus cx-22 microscope and an OptiLab camera with a magnification of 100x40. The data were descriptively analyzed to describe the images of mitotic phases and the mitotic index presentation in the root cells of pigeon pea. The results of this research showed that the cell division of the pigeon pea roots began at 08.00 WIT, which was marked by the presence of a lot of prophase. The next phases that appeared were prometaphase, metaphase, and anaphase which occurred from 08.15 to 08.30 with different numbers. The highest mitotic index occurred at 08.15, when most of the root cells underwent metaphase. This study succeeded in revealing that the optimum time for pigeon pea root cell division is 08.15 WIT. In the future, this research can help pigeon pea farmers in Southwest of Maluku to carry out vegetative reproduction which is closely related to this mitotic study.
Highlights
Pigeon pea (Cajanus cajan) is one of the biodiversity of legumes from Kisar Island, Southwest Maluku, in addition to peanuts, red kidney beans, green beans, and cowpeas
The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells (C. cajan)
The cutting time affects the duration of mitosis and the mitotic index (Yadav, 2007)
Summary
Pigeon pea (Cajanus cajan) is one of the biodiversity of legumes from Kisar Island, Southwest Maluku, in addition to peanuts, red kidney beans, green beans, and cowpeas. The term pigeon pea appeared in the 15th century, originating from America because the seeds were favored by pigeons (Upadhyaya et al, 2015) Nowadays this plant is only consumed as a vegetable and used as a side crop, when compared to other types of plants grown by the people on Kisar Island. Pigeon pea contains several nutrients such as 20-22% protein, 1.2-4.43% fat, 65% carbohydrates, 7.16% fiber, and 3.76-3.80% ash (Sharma et al, 2011; Akande et al, 2010). It contains several kinds of vitamins such as thiamine, riboflavin, and niacin; minerals such as potassium, phosphorus, iron and zinc, calcium, manganese, and copper (Adepoju et al, 2019). The research by Deeplanaiki et al (2013) reported that the cultivar pigeon pea Asha (ICPL 87119) has a DLP gene that determines its function in environmental stress tolerance
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biosaintifika: Journal of Biology & Biology Education
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
